Which of the following statements best justifies the inclusion of test tube V as a control experiment?

24.Amylase is a protein that catalyzes the conversion of starch to simple sugars. Amylase activity in an aqueoussolution can be measured by using iodine as a starch indicator. A solution containing iodine and starch will havea dark-blue color, whereas a solution containing iodine but no starch will have a light-brown color. The colorchange of an iodine solution from dark blue to light brown can be used to measure the rate at which starch isconverted to simple sugars. A student designs an experiment to investigate the effect of environmental pH onamylase function. The design of the experiment is presented in Table 1.Table 1. An experiment for investigating the effect of on amylase functionWhich of the following statements best justifies the inclusion of test tube V as a control in the experiment?(A)It will provide a measurement of amylase activity at an acidic pH.(B)It will provide a measurement of amylase activity at a basic pH.(C)It will show the color change that occurs in the absence of enzyme activity.(D)It will show the color change that occurs in the absence of the amylase protein.