What would happen if lysosomes in cells were to burst?

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PMCA4 deficiency changes the morphology of TNF-α-induced cell death in L929 cells. (A) Parental and PMCAmut L929 cells were untreated (−) or treated with 100 ng of TNF-α/ml (+) for 10 h. Cells were analyzed by microscopy. The genomic DNA was isolated and electrophoresed on a 2% agarose gel. Parental cells died with swelling (Sw), while PMCAmut cells were shrunken (Sh). DNA laddering was detected in TNF-α-treated PMCAmut cells but not in parental cells. (B) Parental, PMCAmut, PMCA4-reconstituted, and empty vector-transfected PMCAmut cells were untreated (−) or treated with TNF-α (+) for 10 h, and the cells were analyzed by PI exclusion plus FSC. The samples were analyzed by flow cytometry (FACSan flow cytometer [Becton Dickinson]) with CellQuest acquisition and analysis software. Different populations of dead and live cells were gated as R1, R2, and R3. R1 cells, necrotic cells that lost plasma membrane integrity, as indicated by PI-positive stains; R2 cells, apoptotic cells that had reduced size and low level of PI stains; R3 cells, healthy cells of normal size with PI-negative stains. The percentages of these three groups of cells are marked. (C) Parental and PMCAmut cells were treated with TNF-α for various times and analyzed as described for panel B. The percentages of R1 and R2 cells are shown. (D) Parental and PMCAmut cells were treated with staurosporine (1 μM), etoposide (10 μM), or lonidamine (10 μM) for 24, 24, and 4 h, respectively, and analyzed as described for panel B. The percentages of R1 and R2 cells are shown.