Magnification power of microscope formula

Magnification:
Magnification power of microscope formula
  • Figuring Total Magnification
Magnifying Objects/ Focusing Image:
  1. When viewing a slide through the microscope make sure that the stage is all the way down and the 4X scanning objective is locked into place.
  2. Place the slide that you want to view over the aperture and gently move the stage clips over top of the slide to hold it into place.
  3. Beginning with the 4X objective, looking through the eyepiece making sure to keep both eyes open (if you have trouble cover one eye with your hand) slowly move the stage upward using the coarse adjustment knob until the image becomes clear. This is the only time in the process that you will need to use the coarse adjustment knob. The microscopes that you will be using are parfocal, meaning that the image does not need to be radically focused when changing the magnification.
  4. To magnify the image to the next level rotate the nosepiece to the 10X objective. While looking through the eyepiece focus the image into view using only the fine adjustment knob, this should only take a slight turn of the fine adjustment knob to complete this task.
  5. To magnify the image to the next level rotate the nosepiece to the 40X objective. While looking through the eyepiece focus the image into view using only the fine adjustment knob, this should only take a slight turn of the fine adjustment knob to complete this task.
Total Magnification:
To figure the total magnification of an image that you are viewing through the microscope is really quite simple. To get the total magnification take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X.
Magnification power of microscope formula
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Answer

Magnification power of microscope formula
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Hint: A microscope is an optical instrument which is used to see small objects like molecules of a substance or cells of an organism. Use the formula for the magnifying power as figure out what should be the condition on the focal lengths of the lenses for increasing the magnifying power.
Formula used:$m=\dfrac{{{v}_{0}}-{{f}_{0}}}{{{f}_{0}}}\left( 1+\dfrac{D}{{{f}_{e}}} \right)$

Complete answer:

A microscope is an optical instrument which is used to see small objects like molecules of a substance or cells of an organism. A microscope magnifies the object or specimen, which helps us to see the small object more clearly and we can analyse it easily. A microscope uses lenses to magnify the object. A compound microscope consists of two convex lenses called objective lens and eye lens.For every microscope, we defined something called the magnifying power (m) of the microscope. For a compound microscope, its magnifying power is given as $m=\dfrac{{{v}_{0}}-{{f}_{0}}}{{{f}_{0}}}\left( 1+\dfrac{D}{{{f}_{e}}} \right)$….. (i).Here, ${{v}_{0}}$ is the distance of the object from the objective lens, ${{f}_{0}}$ and ${{f}_{e}}$ are the focal lengths of the objective lens and the eye lens respectively and D is the least distance of distinct vision.From equation (i) we understand that the value of m will increase when we reduce the denominator. This implies that the values of ${{f}_{0}}$ and ${{f}_{e}}$ must be small. Therefore, the magnifying power of a compound microscope can be increased by using both lenses of smaller focal lengths.

Hence, the correct option is C.

Note:

Total magnification produced by a system of lenses is equal to the product of the magnifications produced by individual lenses.The magnifying power of a compound microscope is also equal to the product of the magnification produced by the objective lens and magnification produced by the eye lens. i.e. $m={{m}_{o}}\times {{m}_{e}}$.

Compound light microscopes magnify objects by using a system of lenses and a light source. They are commonly employed to research bacteria, single cells, and various cell components. You will need to know the power of the ocular and objective lenses to figure out how much your microscope can magnify. The ocular lens is set up in the eyepiece, and objective lenses are universal across all microscopes. 1-4 objective lenses are frequently found on a revolving wheel above the platform on compound microscopes. The overall magnification is calculated by multiplying the ocular and objective lens powers.

How to Calculate the Magnification of a Microscope?

Check for the magnification power of the ocular lens. You can find it marked on the outside of the eyepiece, otherwise, you can look in the manual. The ocular lens usually magnifies 10 times. Next, look for the magnification capacity of your objective lens. You can find this on the side of the lens, or the manual can help. Traditionally the value can vary among 4x, 10x, 40x, 100x.

As the objective lens is the first one to magnify, it is located on a rotating wheel just above the stage where you place the specimen slide. The budget-friendly microscopes might come with only one lens, but the quality ones generally provide you the benefit to adjust among three to four lenses. Microscopes India is the best place to buy microscopes in India.

The total magnification of a microscope = magnification power of the ocular lens x magnification power of the objective lens.

Considering an objective lens of power 40x and the fact that the ocular lens generally magnifies up to 10 times, the total magnification would be 400x. The total magnification a compound light microscope can provide is 1000x.

How to Calculate Field of View Microscope?

For calculating the field of view, find out the magnification power and the field number of the lens you are using. Dividing the field number by the magnification power gives you the diameter of the microscope’s field of view.

The microscope itself tells you every detail you require. When you check the eyepiece, you will see a sequence of numbers labeled in the form 10x/22 or 30x/18. These numbers imply the magnification power and the field number, respectively. You will also require the magnification power of the objective lens located at the bottom of the microscope. They usually mark this as 4, 10, 40, or 100 times.

Once you have all the information about eyepiece magnification, field number, and objective lens magnification sorted, it becomes easier to calculate the microscope’s field of view. Divide the field number by the magnification number. For instance, if the eyepiece is labeled as 30x/18, then 18 ÷ 30 = 0.6, meaning that the diameter of for is 0.6 millimeters.

For microscopes that only have an eyepiece, this will be enough. But having an objective lens as well makes the calculation harder. You must multiply the eyepiece magnification with the objective magnification and then divide the field number. If the eyepiece states 10x/18 and the magnification of the objective lens is 40, then the total magnification will be 10 x 40 = 400. After 18 ÷ 400, the diameter comes to 0.045 millimeters. To convert the measurements from millimeters to micrometers, divide the diameter by 1000.

Why Is It Important to Calculate the Diameter of the Field When First Using the Microscope?

The field diameter is the viewing area of the lens of the microscope. It is the only number of millimeters or micrometers of the area that you can see. By measuring the field diameter, you can calculate the real size of the objects that are too small to measure.

Determining the Best Microscope for Laboratory Use

The best laboratory microscope manufacturer and supplier in India is microscopes India. They sell the only premium quality with the latest technology.

Microscope Type

What will make your choice easy is determining the kind and size of the specimen you will be studying.

Compound Microscope

The word “microscope” automatically takes our brains to compound microscopes. The specimens can be placed on slides, zoomed in for higher magnifications and tiny specimens become easier to study. Biological microscopes are often used to observe specimens of cells and organisms found in pond water. The metallurgical microscopes are great to study cross-sections of circuit boards and polarizing microscopes are built to study thin sections of rock.

Stereo Microscope

These are used to study objects that have a decent size such as leaves, flowers, insects, gems, rocks, etc. Stereo microscopes allow moderate magnification and are easy to handle. Production facilities often pick up these kinds of microscopes and even manufacturing plants that need to go through immediate inspection. Students are handed stereo microscopes when they are just starting out.

Digital Microscope

It is the new age microscope with a camera and eyepiece attached to the microscope head. The view through the lens is projected on an HDMI screen or a pc monitor. You get high-quality images of slides as well as a larger specimen.

Microscope Lighting

Lighting plays an important role to make the microscope portable for field research. It must concentrate on the specimen to study the details appropriately. There are both top and bottom illumination available for some microscopes but in general, the light is located at the bottom. There are microscopes built with cool led lighting, keeping in mind that overheating might damage sensitive slides.

To achieve a greater magnification of an object you must check that the working distance is large. The working distance of a microscope is the gap between the slide and the bottom of the microscope lens. On increasing the magnification, the walking distance reduces. This makes it hard to work under the microscope. There are some special microscopes that are designed to provide extended working distances so that the zooming range is not compromised.

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